Laboratory Report

Results

Effect of Fiber on Transit Time - Number of Markers in Stomach
	Standard Diet			Insoluble Fiber-Rich Diet			Soluble Fiber-Rich Diet
	Minutes After Markers Ingestion. Number of Markers Present.			Minutes After Markers Ingestion. Number of Markers Present.			Minutes After Markers Ingestion. Number of Markers Present.
Subject	60 min	180 min	240 min	60 min	180 min	240 min	60 min	180 min	240 min
1
2
3
Average		Minimum 3 characters, two numbers, one digital full stop. The RV(L) value is valid, thank you. Sorry. Your value is wrong. Please try again. The valid RV(L) value is correct, thank you.			Minimum 3 characters, two numbers, one digital full stop. The RV(L) value is valid, thank you. Sorry. Your value is wrong. Please try again. The valid RV(L) value is correct, thank you.			Minimum 3 characters, two numbers, one digital full stop. The RV(L) value is valid, thank you. Sorry. Your value is wrong. Please try again. The valid RV(L) value is correct, thank you.
Average Percentage of The Total Markers		Minimum 3 characters, two numbers, one digital full stop. The RV(L) value is valid, thank you. Sorry. Your value is wrong. Please try again. The valid RV(L) value is correct, thank you.			Minimum 3 characters, two numbers, one digital full stop. The RV(L) value is valid, thank you. Sorry. Your value is wrong. Please try again. The valid RV(L) value is correct, thank you.			Minimum 3 characters, two numbers, one digital full stop. The RV(L) value is valid, thank you. Sorry. Your value is wrong. Please try again. The valid RV(L) value is correct, thank you.

Food Transition Through the Stomach

For the standard meal, what is the average percentage of markers remaining in the stomach at 60 min, 180 min, and 240 min?
For the meal with insoluble fibers, what is the average percentage of markers remaining in the stomach at 60 min, 180 min, and 240 minutes?
For the meal with soluble fibers, what is the average percentage of markers remaining in the stomach at 60 min, 180 min, and 240 min?
Which meal had the greatest average percentage of markers in the stomach at 180 minutes?

Effect of Fiber on Transit Time - Number of Markers in Large Bowel
Food	Subject	RC			LC			SC			Total (RC+LC+SC)
		Hours After Markers Ingestion. Number of Markers Present.			Hours After Markers Ingestion. Number of Markers Present.			Hours After Markers Ingestion. Number of Markers Present.			Hours After Markers Ingestion. Number of Markers Present.
		24 hours	48 hours	96 hours	24 hours	48 hours	96 hours	24 hours	48 hours	96 hours	24 hours	48 hours	96 hours
Standard Diet	1										Minimum 3 characters, two numbers, one digital full stop. The RV(L) value is valid, thank you. Sorry. Your value is wrong. Please try again. The valid RV(L) value is correct, thank you.
	2
	3
	Average										Minimum 3 characters, two numbers, one digital full stop. The RV(L) value is valid, thank you. Sorry. Your value is wrong. Please try again. The valid RV(L) value is correct, thank you.
	Average Percentage of The Total Markers										Minimum 3 characters, two numbers, one digital full stop. The RV(L) value is valid, thank you. Sorry. Your value is wrong. Please try again. The valid RV(L) value is correct, thank you.
Insoluble Fiber-Rich Diet	1										Minimum 3 characters, two numbers, one digital full stop. The RV(L) value is valid, thank you. Sorry. Your value is wrong. Please try again. The valid RV(L) value is correct, thank you.
	2
	3
	Average										Minimum 3 characters, two numbers, one digital full stop. The RV(L) value is valid, thank you. Sorry. Your value is wrong. Please try again. The valid RV(L) value is correct, thank you.
	Average Percentage of The Total Markers										Minimum 3 characters, two numbers, one digital full stop. The RV(L) value is valid, thank you. Sorry. Your value is wrong. Please try again. The valid RV(L) value is correct, thank you.
Soluble Fiber-Rich Diet	1										Minimum 3 characters, two numbers, one digital full stop. The RV(L) value is valid, thank you. Sorry. Your value is wrong. Please try again. The valid RV(L) value is correct, thank you.
	2
	3
	Average										Minimum 3 characters, two numbers, one digital full stop. The RV(L) value is valid, thank you. Sorry. Your value is wrong. Please try again. The valid RV(L) value is correct, thank you.
	Average Percentage of The Total Markers										Minimum 3 characters, two numbers, one digital full stop. The RV(L) value is valid, thank you. Sorry. Your value is wrong. Please try again. The valid RV(L) value is correct, thank you.

Food Transition Through the Large Bowel

For the standard meal, what is the average percentage of markers in the right colon (RC), left colon (LC), sigmoid colon (SC) and total colon at 24, 48, and 96 hours?
For the meal with insoluble fibers, what is the average percentage of markers in the right colon (RC), left colon (LC), sigmoid colon (SC) and total colon at 24, 48, and 96 hours?
For the meal with soluble fibers, what is the average percentage of markers in right colon (RC), left colon (LC), sigmoid colon (SC) and total colon at 24, 48, and 96 hours?
Which meal had the least percentage of markers in the total colon at 24 hours?

Effect of Dietary Fiber on Bile Acid Adsorption
Experimental Trials	Optical density (OD) of colored filtrate (reflects amount of bile acid in filtrate)					1 - OD (reflects amount of bile acid adsorbed by food particles)
Experimental Trials	Bile Acid With Buffer Solution (No Fibers	Standard Meal Without Bile	Standard Meal With Bile Acid	Fiber-Rich Meal Without Bile	Fiber-Rich Meal With Bile Acid	Bile Acid With Buffer Solution (No Fibers	Standard Meal Without Bile	Standard Meal With Bile Acid	Fiber-Rich Meal Without Bile	Fiber-Rich Meal With Bile Acid
1								Minimum 3 characters, two numbers, one digital full stop. The RV(L) value is valid, thank you. Sorry. Your value is wrong. Please try again. The valid RV(L) value is correct, thank you.		Minimum 3 characters, two numbers, one digital full stop. The RV(L) value is valid, thank you. Sorry. Your value is wrong. Please try again. The valid RV(L) value is correct, thank you.
2
3
Average			Minimum 3 characters, two numbers, one digital full stop. The RV(L) value is valid, thank you. Sorry. Your value is wrong. Please try again. The valid RV(L) value is correct, thank you.		Minimum 3 characters, two numbers, one digital full stop. The RV(L) value is valid, thank you. Sorry. Your value is wrong. Please try again. The valid RV(L) value is correct, thank you.			Minimum 3 characters, two numbers, one digital full stop. The RV(L) value is valid, thank you. Sorry. Your value is wrong. Please try again. The valid RV(L) value is correct, thank you.		Minimum 3 characters, two numbers, one digital full stop. The RV(L) value is valid, thank you. Sorry. Your value is wrong. Please try again. The valid RV(L) value is correct, thank you.

Effect of Dietary Fibers on Bile Acid Adsorption

Which had the greatest average OD (amount of bile acid in filtrate), the standard meal with bile acid or the fiber-rich meal with bile acid?
Which had the greatest average 1- OD (amount of bile acid adsorbed to meal particles), the standard meal with bile acid or the fiber-rich meal with bile acid?

Effect of Dietary Fiber on Transit Time and Bile April 10, 2013

1. Dietary fiber will: undefined 2. Dietary fiber will undefined Effect of Dietary Fiber on Transit Time 1. Dependent Variable undefined 2. Independent Variable undefined 3. Controlled Variables undefined; undefined Effect of Dietary Fiber on Bile Acid Adsorption 1. Dependent Variable undefined 2. Independent Variable undefined 3. Controlled Variables undefined; undefined 4. Name the type of method used to measure transit time. undefined 5. Name the type of method used to measure the amount of bile acids in solution. undefined Table 3. Effect of Fiber on Transit Time - Number of Markers in Stomach

Standard diet

Insoluble fiber-rich diet

Soluble fiber-rich diet

Minutes after markers ingestion.

Number of markers present.

Minutes after markers ingestion.

Number of markers present.

Minutes after markers ingestion.

Number of markers present.

60 min

180 min

240 min

60 min

180 min

240 min

60 min

180 min

240 min

Food Transition Through the Stomach Standard diet Insoluble fiber-rich diet Soluble fiber-rich diet 0 20 40 60 80 100 60 120 180 240 60 180 240 1. For the standard meal, what is the average percentage of markers remaining in the stomach at 60 min, 180 min, and 240 min? undefined 2. For the meal with insoluble fibers, what is the average percentage of markers remaining in the stomach at 60 min, 180 min, and 240 minutes? undefined 3. For the meal with soluble fibers, what is the average percentage of markers remaining in the stomach at 60 min, 180 min, and 240 min? undefined 4. Which meal had the greatest average percentage of markers in the stomach at 180 minutes? undefined Table 4. Effect of Fiber on Transit Time - Number of Markers in Large Bowel

Food

Subject

Total (RC+LC+SC)

Hours after markers ingestion. Number of markers present.

24 hours

48 hours

96 hours

24 hours

48 hours

96 hours

24 hours

48 hours

96 hours

24 hours

48 hours

96 hours

Food Transition Through the Large Bowel Standard diet Insoluble fiber-rich diet Soluble fiber-rich diet 0 20 40 60 80 100 24 48 72 96 24 48 96 5. For the standard meal, what is the average percentage of markers in the right colon (RC), left colon (LC), sigmoid colon (SC) and total colon at 24, 48, and 96 hours? undefined 6. For the meal with insoluble fibers, what is the average percentage of markers in the right colon (RC), left colon (LC), sigmoid colon (SC) and total colon at 24, 48, and 96 hours? undefined 7. For the meal with soluble fibers, what is the average percentage of markers in right colon (RC), left colon (LC), sigmoid colon (SC) and total colon at 24, 48, and 96 hours? undefined 8. Which meal had the least percentage of markers in the total colon at 24 hours? undefined Table 5. Effect of Dietary Fiber on Bile Acid Adsorption

Experimental Trials

Optical density (OD) of colored filtrate (reflects amount of bile acid in filtrate)

1 - OD (reflects amount of bile acid adsorbed by food particles)

Bile Acid With Buffer Solution (No Fibers)

Standard meal without bile

Standard meal with bile acid

Fiber-Rich Meal Without Bile

Fiber-rich meal with bile acid

Bile Acid With Buffer Solution (No Fibers)

Standard meal without bile

Standard meal with bile acid

Fiber-Rich Meal Without Bile

Fiber-rich meal with bile acid

Effect of Dietary Fibers on Bile Acid Adsorption Averaged - OD Averaged - (1-OD) 0 0.2 0.4 0.6 0.8 1 Standard Meal Fiber-Rich Meal 9. Which had the greatest average OD (amount of bile acid in filtrate), the standard meal with bile acid or the fiber-rich meal with bile acid? undefined 10. Which had the greatest average 1- OD (amount of bile acid adsorbed to meal particles), the standard meal with bile acid or the fiber-rich meal with bile acid? undefined 1. Approximately how long did it take for a meal to move through the stomach, less than 60 minutes, between 60 and 180 minutes, or between 180 and 240 minutes? undefined 2. Which meal spent the most time in the stomach? Explain your choice based on your results. undefined 3. Approximately how long did it take for a meal to move through the colon for the standard diet, the soluble fiber-rich diet, and insoluble fiber-rich diet? undefined 4. Which meal spent the least time in the colon? Explain your choice based on your results. undefined 5. Based on the average number of markers in the right colon and the average total number of markers in the colon at 24 hours, which meal do you think spent the least amount time in the small intestine? Explain your choice based on your results undefined 6. Does fiber increase or decrease the amount of bile lost in feces? Explain your choice based on your results. undefined 1. Explain why eating a high fiber diet may reduce your serum cholesterol level. undefined 2. Based on your results, speculate why eating a high fiber diet may prevent large changes in blood glucose levels. undefined