Laboratory Report

Materials and Methods

Why was RIA used to measure Thyroxine while IRMA was used to measure TSH?
How was it used to observe relative amount of iodine accumulated by thyroid gland?

Results

Concentration of Thyroxine (T₄) and Thyroid Stimulating Hormone (TSH) in Blood Serum
	Normal	Patient 1		Patient 2		Patient 3
	Normal	Value	Status	Value	Status	Value	Status
T₄ (mg/l)	0.05 ÷ 0.14		Select Concentration		Select Concentration		Select Concentration
TSH (mlU/I)	0.35 ÷ 4.5		Select Concentration		Select Concentration		Select Concentration
Thyroid Image			Select		Select		Select
Type of Thyroid Disorder		Select		Select		Select

The normal range for total amount of T₄ in serum is 0.05-0.14 mg/L For each patient, type in their serum T₄ concentration and indicate if it is lower, within, or higher than the normal range.
1. Patient with primary hypothyroidism:
2. Patient with secondary hypothyroidism:
3. Patient with hyperthyroidism:
The normal range for serum TSH is 0.35-4.5 mlU/L. For each patient, type in her serum TSH concentration and indicate if it is lower, within, or higher than the normal range.
1. Patient with primary hypothyroidism:
2. Patient with secondary hypothyroidism:
3. Patient with hyperthyroidism:
For each patient, state whether her thyroid image indicates whether their iodine uptake is low, normal, or high.
1. Patient with primary hypothyroidism:
2. Patient with secondary hypothyroidism:
3. Patient with hyperthyroidism:

Discussion

For each subject, indicate whether her TRH levels would be low, normal, or high. Please explain your decision. Discuss negative feedback control in your answer.
1. Subject with primary hypothyroidism:
2. Subject with secondary hypothyroidism:
3. Subject with hyperthyroidism:
For each subject, explain why iodine uptake would be greater or less than normal.
1. Subject with primary hypothyroidism:
2. Subject with secondary hypothyroidism:
3. Subject with hyperthyroidism:
State how hypothyroidism affects each of the following and describe the mechanism by which this change occurs.
1. body temperature:
2. heart rate:
State how hyperthyroidism affects each of the following and describe the mechanism by which this change occurs.
1. body temperature:
2. heart rate:
Restate your predictions that were correct and give the data from your experiment that supports them. Restate your predictions that were not correct and correct them, giving the data from your experiment that supports the corrections,:

Application

The subject who was diagnosed with primary hypothyroidism was given levothyroxine (synthetic T₄). After 2 months of treatment, her TSH values decreased by 96.38 % and her T₄ levels increased by 879.4 %. Explain why her TSH levels decreased and T₄ increased.
The subject who was diagnosed with secondary hypothyroidism was given levothyroxine (synthetic T₄). After 6 weeks of treatment, her TSH values did not change and her T₄ levels increased by 778.62 %. Explain the why TSH did not change and why T₄ increased.
The subject who was diagnosed with hyperthyroidism was given propylthiouracil (prevents T₄ production and conversion of T₄ to T₃ in the tissues). After 2 months of treatment, her TSH levels increased by 371.15 % and her T₄ levels decreased by 42.7 %. Explain why her TSH levels increased and her T₄ levels decreased.

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Homeostatic Imbalances of Thyroid Function February 12, 2013

1. TSH levels in patients with primary hypothyroidism are: undefined 2. Thyroxine levels in patients with primary hypothyroidism are: undefined 3. TSH levels in patients with secondary hypothyroidism are: undefined 4. Thyroxine levels in patients with secondary hypothyroidism are: undefined 5. TSH levels in patients with hyperthyroidism are: undefined 6. Thyroxine levels in patients with hyperthyroidism are: undefined 1. Name the Dependent Variable. undefined 2. Name the Independent Variable. undefined 3. Name the Controlled Variables. undefined; undefined 4. Why was RIA used to measure Thyroxine while IRMA was used to measure TSH? undefined 5. How was it used to observe relative amount of iodine accumulated by thyroid gland? undefined Table 2. Concentration of Thyroxine and Thyroid Stimulating Hormone (TSH) in Blood Serum

Normal

Patient 1

Patient 2

Patient 3

Value

Status

Value

Status

Value

Status

1. The normal range for total amount of Thyroxine in serum is 0.05 - 0.14 mg/L. For each patient, type in their serum Thyroxine concentration and indicate if it is lower, within, or higher than the normal range. a. Patient with primary hypothyroidism undefined b. Patient with secondary hypothyroidism undefined c. Patient with hyperthyroidism undefined 2. The normal range for serum TSH is 0.35 - 4.5 mIU/L . For each patient, type in her serum TSH concentration and indicate if it is lower, within, or higher than the normal range. a. Patient with primary hypothyroidism undefined b. Patient with secondary hypothyroidism undefined c. Patient with hyperthyroidism undefined 3. For each patient, state whether her thyroid image indicates whether their iodine uptake is low, normal, or high. a. Patient with primary hypothyroidism undefined b. Patient with secondary hypothyroidism undefined c. Patient with hyperthyroidism undefined 1. For each subject, indicate whether her TRH levels would be low, normal, or high. Please explain your decision. Discuss negative feedback control in your answer. a. Subject with primary hypothyroidism undefined b. Subject with secondary hypothyroidism undefined c. Subject with hyperthyroidism. undefined 2. For each subject, explain why iodine uptake would be greater or less than normal. a. Subject with primary hypothyroidism undefined b. Subject with secondary hypothyroidism undefined c. Subject with hyperthyroidism. undefined 3. State how hypothyroidism affects each of the following and describe the mechanism by which this change occurs. a. body temperature undefined b. heart rate undefined 4. State how hyperthyroidism affects each of the following and describe the mechanism by which this change occurs. a. body temperature undefined b. heart rate undefined 5. Restate your predictions that were correct and give the data from your experiment that supports them. Restate your predictions that were not correct and correct them, giving the data from your experiment that supports the corrections. undefined 1. The subject who was diagnosed with primary hypothyroidism was given levothyroxine (synthetic Thyroxine). After 2 months of treatment, her TSH values decreased by 96.38% and her Thyroxine levels increased by 879.4%. Explain why her TSH levels decreased and Thyroxine increased. undefined 2. The subject who was diagnosed with secondary hypothyroidism was given levothyroxine (synthetic Thyroxine). After 6 weeks of treatment, her TSH values did not change and her Thyroxine levels increased by 778.62%. Explain the why TSH did not change and why Thyroxine increased. undefined 3. The subject who was diagnosed with hyperthyroidism was given propylthiouracil (prevents Thyroxine production and conversion of Thyroxine to T3 in the tissues). After 2 months of treatment, her TSH levels increased by 371.15% and her Thyroxine levels decreased by 42.7%. Explain why her TSH levels increased and her Thyroxine levels decreased. undefined