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Congratulations on completing Pre-Lab Reporting.

Select Data Collection to continue the experiment.

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Synopsis of Experiment

A thin slice of rat brain from the hippocampus will be obtained in order to observe multipolar neurons.

To determine the location of the trigger zone, a neuron will be filled with a voltage-dependent fluorescent dye. The dendrite will be stimulated with an electric charge (measured in volts) large enough to generate an action potential. Changes in membrane potential will be observed as color changes and the color change due to an action potential will first be observed at the trigger zone (continue scrolling for more).

Experiment 1: Effect of Stimulus Strength on Action Potential Generation

Stimulate the dendrite with increasing voltages (representing increasing graded potential voltages) until an action potential is generated. Patch clamp electrodes and voltage-dependent fluorescent dye will be used to measure the action potential voltage at the axon hillock and axon.

Experiment 2: Effect of Frequency of Stimulation on Action Potential Generation

Stimulate dendrite at increasing frequencies (less time between stimulations). Measure the number of action potentials generated.

Predictions

Based on what you know, predict the outcome of each experiment. Complete the numbered statements below by choosing one response for each number.

When you are done, click the Submit button to save your responses to the laboratory report.

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Your Predictions have been submitted to the Lab Report.

Scroll down to select Variables.

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You have correctly defined all of the variables.

Click or tap the Submit to Lab Report button to continue.

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Correct.

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Try Again.

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You have not defined all of the variables correctly. Try again.

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Variables

Now determine the dependent, independent, and controlled variables for each experiment in this laboratory activity by clicking on the appropriate answers.

Check your answers until you have chosen all variables correctly. There will be one choice for Dependent and Independent Variables and multiple choices for Controlled Variables. When you are done, click the Submit button to save your responses to the laboratory report.

Submit to Lab Report

Your data for the entire module, including the Laboratory Report, will be deleted.

Data Collection will refresh with a new data set.

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